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KMID : 0903519950380060490
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 6 p.490 ~ p.495
Production of Cellulase from Cellulomonas sp . KL - 6


Abstract
Among the cellulases by Celludomonas sp. KL-6. CMCase and filter paperase, which were produced as the out enzymes of cell, had been much produced, but very small amounts of ¥â-glucosidase, the enzyme of which is cell bound form, was produced by this organism. The optimal culture times for CMCase and filter paperase productions were 5 days, while that of ¥â-glucosidase was 4 days. When this strain was cultured under the optimal medium for enzyme production, CMCase, FPase and ¥â-glucosidase were 82 units/§¢. 80 units/§¢ and 1.2 units/§¢, respectively. Thus these results were showed to increase enzyme productivities as about 60¡­70% than those produced in basal medium. CaCO©ý injected to the medium as the ratio of 0.1% was not only enhanced cellulase activities but also effective as acid neutralizing agent. The production effects of lignase and lactase by this bacterium in filter paper medium was not appeared.
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